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Mutation in the glmS gene controlling cell wall synthesis increases resistance to the herbicide amitrole in cyanobacterium Synechocystis sp. PCC6803статья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Babykin M.M., Kovach A., Cerff R., Shestakov S.V.
- Журнал: Russian Journal of Genetics
- Том: 36
- Номер: 10
- Год издания: 2000
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 1181
- Последняя страница: 1184
- Аннотация: A DNA fragment transforming cells of the cyanobacterium Synechocystis sp. PCC6803 to amitrole (3-amino-1,2,4-triazole) resistance was cloned from cells of the Atr2 mutant resistant to this herbicide. The transforming activity of the cloned fragment was shown to be associated with the missense mutation Val250-Leu250 in the glmS gene encoding glucosamine-6-phosphate synthase, the key enzyme of cell wall synthesis. The amino acid substitution is located in the central, nonconservative region of the GlmS protein, far from the two reaction centers positioned at the polypeptide ends. It is suggested that the mutant protein has lost sensitivity to amitrole. In the wild-type strain, this herbicide causes conditional glucosamine auxotrophy (exogenous glucosamine restores cell ability to grow in the presence of lethal amitrole concentrations). It is proposed that Val250 is an allosteric binding site of the herbicide in the GlmS protein of the cyanobacterium.
- Добавил в систему: Бабыкин Михаил Михайлович