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3D or not 3D: a guide to assess cell viability in 3D cell systemsстатья
- Авторы: Bikmulina Polina, Kosheleva Nastasia, Efremov Yuri, Antoshin Artem, Heydari Zahra, Kapustina Valentina, Royuk Valery, Mikhaylov Vasily, Fomin Victor, Vosough Massoud, Timashev Peter, Rochev Yury, Shpichka Anastasia
- Журнал: Soft Matter
- Том: 18
- Номер: 11
- Год издания: 2022
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 2222
- Последняя страница: 2233
- DOI: 10.1039/d2sm00018k
- Аннотация: Cell viability is the primary integrative parameter used for various purposes, particularly when fabricating tissue equivalents (e.g., using bioprinting or scaffolding techniques), optimizing conditions to cultivate cells, testing chemicals, drugs, and biomaterials, etc. Most of the conventional methods were originally designed for a monolayer (2D) culture; however, 2D approaches fail to adequately assess a tissue-engineered construct's viability and drug effects and recapitulate the host–pathogen interactions and infectivity. This study aims at revealing the influence of particular 3D cell systems’ parameters such as the components’ concentration, gel thickness, cell density, etc. on the cell viability and applicability of standard assays. Here, we present an approach to achieving adequate and reproducible results on the cell viability in 3D collagen- and fibrin-based systems using the Live/Dead, AlamarBlue, and PicoGreen assays. Our results have demonstrated that a routine precise analysis of 3D systems should be performed using a combination of at least three methods based on different cell properties, e.g. the metabolic activity, proliferative capacity, morphology, etc.
- Добавил в систему: Тимашев Петр Сергеевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | 2022_3D_or_not_3D_-_a_guide_to_assess_cell_viability_in_3D_… | 4,2 МБ | 3 августа 2022 [TPS_SU] |