Honeycomb-Structured Porous Films from Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Physicochemical Characterization and Mesenchymal Stem Cells Behavior

Kulikouskaya, V.I.; Nikalaichuk, V.V.; Bonartsev, A.P.; Akoulina, E.A.; Belishev, N.V.; Demianova, I.V.; Chesnokova, D.V.; Makhina, T.K.; Bonartseva, G.A.; Shaitan, K.V.; Hileuskaya, K.S.; Voinova, V.V.

Авторы: Kulikouskaya Viktoryia I., Nikalaichuk Viktoryia V., Bonartsev Anton P., Akoulina Elizaveta A., Belishev Nikita V., Demianova Irina V., Chesnokova Dariana V., Makhina Tatiana K., Bonartseva Garina A., Shaitan Konstantin V., Hileuskaya Kseniya S., Voinova Vera V.
Журнал: Polymers
Том: 14
Номер: 13
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 2671
DOI: 10.3390/polym14132671
Аннотация: Surface morphology affects cell attachment and proliferation. In this research, different films made of biodegradable polymers, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrateco-3-hydroxyvalerate) (PHB-co-HV), containing different molecular weights, with microstructured surfaces were investigated. Two methods were used to obtain patterned films—water-assisted self-assembly (“breath figure”) and spin-coating techniques. The water-assisted technique made it possible to obtain porous films with a self-assembled pore structure, which is dependent on the monomer composition of a polymer along with its molecular weight and the technique parameters (distance from the nozzle, volume, and polymer concentration in working solution). Their pore morphologies were evaluated and their hydrophobicity was examined. Mesenchymal stem cells (MSCs) isolated from bone marrow were cultivated on a porous film surface. MSCs’ attachment differed markedly depending on surface morphology. On strip-formed stamp films, MSCs elongated along the structure, however, they interacted with a larger area of film surface. The honeycomb films and column type films did not set the direction of extrusion, but cell flattening depended on structure topography. Thus, stem cells can “feel” the various surface morphologies of self-assembled honeycomb films and change their behavior depending on it.
Добавил в систему: Бонарцев Антон Павлович

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Honeycomb-Structured Porous Films from Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Physicochemical Characterization and Mesenchymal Stem Cells Behaviorстатья Исследовательская статья

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