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Schizophrenia patients have significantly less f-SatIII and more rDNA repeats compared to healthy donorsстатья
- Авторы: Sergeeva V., Poletkina A., Ershova E., Veiko N., Martynov A., Chudakova J., Artyushin A., Malinovskaya E., Filev A., Konkova M., Bravve L., Zakharova N., Kostyuk G., Agafonova O., Shmarina G., Kostyuk S.
- Журнал: European Journal of Human Genetics
- Том: 28
- Номер: S1
- Год издания: 2020
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 893
- Аннотация: Introduction: Dysregulation of rRNA biogenesis is implicated in some human diseases. rDNA copy number (CN) in the genome is а factor at play. F-Sat III is an AT-rich repeat of pericentromeric heterochromatin in the first chromosome (lq l2). rDNA and lq 12 interact in interphase cells. rDNA CN might coпelate with f-Sat III.Materials and methods: W е analyzed leukocytes of 469 healthy donors (HD) and 777 patients with schizophrenia (F20.00, SP). CN was determined using non-radioactive hybridization with biotin-labelled DNA probes.Results: Genomes of SP contain increased levels of rDNA CN and decreased levels of f-Sat-III compared to HD: 532 ± 118 copies with 18,0 ± 5,9 pg/ng of f-Sat III and 413 ± 105 copies with 21,6 ± 6,9 pg/ng of f-Sat III, respectively, р < 10 -80. Amount of f-Sat III depends on rDNA CN:DNA of HD and SP containing low (200 - 350) or high (>700) CN of rDNA contained 10 - 30pg/ng of f-Sat III. 55% of SP have 500 - 600 CN of rDNA and 15-20 pg/ng of f-Sa tIII. Only 15% of HD have the same numbers. 16% of SP have <20 pg/ng of f-Sat III and rDNA CN > 500 with their ratio > 40, only 1.5% of HD have this ratio. 26% of HD have rDNA CN <400, f-Sat III> 20 pg/ng and their ratio <20 and only 1, 9% of SP are in this area. Thus, there is a coпelation between rDNA CN and the size if heterochromatin regions.Conclusions: SP have significantly less f-Sat III and more rDNA repeats compared to HD.
- Добавил в систему: Костюк Георгий Петрович