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Modulation of Membrane Microviscosity by Protein-Mediated Carotenoid Delivery as Revealed by Time-Resolved Fluorescence Anisotropyстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Semenov Alexey N., Gvozdev Danil A., Zlenko Dmitry V., Protasova Elena A., Khashimova Anastasia R., Parshina Evgenia Yu, Baizhumanov Adil A., Lotosh Natalia Yu, Kim Eleonora E., Kononevich Yuriy N., Pakhomov Alexey A., Selishcheva Alla A., Sluchanko Nikolai N., Shirshin Evgeny A., Maksimov Eugene G.
- Журнал: Membranes
- Том: 12
- Номер: 10
- Год издания: 2022
- Издательство: MDPI Publishing
- Местоположение издательства: Basel, Switzerland, Switzerland
- Первая страница: 1
- Последняя страница: 22
- Номер статьи: 905
- DOI: 10.3390/membranes12100905
- Аннотация: Carotenoids are potent antioxidants with a wide range of biomedical applications. How- ever, their delivery into human cells is challenging and relatively inefficient. While the use of natural water-soluble carotenoproteins capable to reversibly bind carotenoids and transfer them into mem- branes is promising, the quantitative estimation of the delivery remains unclear. In the present work, we studied echinenone (ECN) delivery by cyanobacterial carotenoprotein AnaCTDH (C-terminal do- main homolog of the Orange Carotenoid Protein from Anabaena), into liposome membranes labelled with BODIPY fluorescent probe. We observed that addition of AnaCTDH-ECN to liposomes led to the significant changes in the fast-kinetic component of the fluorescence decay curve, pointing on the dipole-dipole interactions between the probe and ECN within the membrane. It may serve as an indirect evidence of ECN delivery into membrane. To study the delivery in detail, we carried out molecular dynamics modeling of the localization of ECN within the lipid bilayer and calculate its orientation factor. Next, we exploited FRET to assess concentration of ECN delivered by AnaCTDH. Finally, we used time-resolved fluorescence anisotropy to assess changes in microviscosity of liposo- mal membranes. Incorporation of liposomes with β-carotene increased membrane microviscosity while the effect of astaxanthin and its mono- and diester forms was less pronounced. At temperatures below 30 ◦C addition of AnaCTDH-ECN increased membrane microviscosity in a concentration- dependent manner, supporting the protein-mediated carotenoid delivery mechanism. Combining all data, we propose FRET-based analysis and assessment of membrane microviscosity as potent approaches to characterize the efficiency of carotenoids delivery into membranes.
- Добавил в систему: Семенов Алексей Николаевич
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | membranes-12-00905.pdf | 4,2 МБ | 20 сентября 2022 [Alsem1989] | |
| 2. | Экспертное заключение | IMG_0001.pdf | 656,9 КБ | 22 сентября 2022 [kononevich.yuriy] |