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A comparative structural analysis of Fab-fragments of monoclonal rheumatoid and non-rheumatoid immunoglobulins Mстатья
Дата последнего поиска статьи во внешних источниках: 14 августа 2017 г.
- Авторы: Timofeev V.P., Tkachev Y.V., Novikov V.V., Varlamova E.Y., Lapuk V.A.
- Журнал: Biophysics
- Том: 50
- Номер: 5
- Год издания: 2005
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 787
- Последняя страница: 792
- Аннотация: The spin-labeling method was used to study the Fab- and Fab-RF-fragments of IgM and IgM-RF, respectively. The spin-label 2,2,6,6-tetramethyl-4-dichloro-sym-triazinyl-aminopiperidine-l-oxyl was introduced into the peptide moiety of the proteins. The rotational correlation time t of the spin-label carrier was determined based on the temperature-viscosity dependence of the EPR spectra parameters of the spin-labeled proteins. The tau values for Fab- and Fab-RF-fragments were 21 +/- 2 and 12 +/- 1 ns, respectively. The data strongly suggest that the significantly lower tau value for the Fab-RF-fragment may be due to the local structural flexibility of the fragment, which in turn may explain the peculiarities of IgM-RF as an autoantibody.
- Добавил в систему: Новиков Валентин Владимирович