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Microfluidic Chip as a Tool for Effective In Vitro Evaluation of Cyclophosphamide Prodrug Toxicityстатья
- Авторы: Pulkova N.V., Zyrina A.N., Mnafki N.A., Kuznetsova I.M.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 173
- Номер: 1
- Год издания: 2022
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 146
- Последняя страница: 150
- DOI: 10.1007/s10517-022-05510-6
- Аннотация: Most drugs are metabolized in the liver, which can lead to their activation or inactivation with a change in the parent compound pharmacology, as well as liver damage by active metabolites. Preclinical animal studies of the drug safety do not always predict the drug effect on humans due to species specificity. Thus, for the rapid drug screening, prodrugs in particular, an in vitro system is required that allows predicting the xenobiotics cytotoxicity, taking into account their metabolism in liver cells. The microfluidic chip (LLC STC "BioClinicum") have made it possible to cultivate a two-dimensional culture of human HaCaT keratinocytes with spheroids of the HepaRG human hepatoma cell line. After the incubation in universal composition of the serum-free medium containing 3.8 mM cyclophosphamide, the clear death of HaCaT cells was observed compared to cultivation in the absence of liver cells.
- Добавил в систему: Зырина Анна Николаевна