|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Allele-Specific Biased Expression of the CNTN6 Gene in iPS Cell-Derived Neurons from a Patient with Intellectual Disability and 3p26.3 Microduplication Involving the CNTN6 Geneстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Gridina Maria M., Matveeva Natalia M., Fishman Veniamin S., Menzorov Aleksei G., Kizilova Helen A., Beregovoy Nikolay A., Kovrigin Igor I., Pristyazhnyuk Inna E., Oscorbin Igor P., Filipenko Maxim L., Kashevarova Anna A., Skryabin Nikolay A., Nikitina Tatyana V., Sazhenova Elena A., Nazarenko Ludmila P., Lebedev Igor N., Serov Oleg L.
- Журнал: Molecular Neurobiology
- Том: 55
- Номер: 8
- Год издания: 2018
- Издательство: Humana Press, Inc.
- Местоположение издательства: United States
- Первая страница: 6533
- Последняя страница: 6546
- DOI: 10.1007/s12035-017-0851-5
- Аннотация: Copy number variations (CNVs) of the human CNTN6 gene caused by megabase-scale microdeletions or microduplications in the 3p26.3 region are often the cause of neurodevelopmental disorders, including intellectual disability and developmental delay. Surprisingly, patients with different copy numbers of this gene display notable overlapping of neuropsychiatric symptoms. The complexity of the study of human neuropathologies is associated with the inaccessibility of brain material. This problem can be overcome through the use of reprogramming technologies that permit the generation of induced pluripotent stem (iPS) cells from fibroblasts and their subsequent in vitro differentiation into neurons. We obtained a set of iPS cell lines derived from a patient carrier of the CNTN6 gene duplication and from two healthy donors. All iPS cell lines displayed the characteristics of pluripotent cells. Some iPS cell lines derived from the patient and from healthy donors were differentiated in vitro by exogenous expression of the Ngn2 transcription factor or by spontaneous neural differentiation of iPS cells through the neural rosette stage. The obtained neurons showed the characteristics of mature neurons as judged by the presence of neuronal markers and by their electrophysiological characteristics. Analysis of allele-specific expression of the CNTN6 gene in these neuronal cells by droplet digital PCR demonstrated that the level of expression of the duplicated allele was significantly reduced compared to that of the wild-type allele. Importantly, according to the sequencing data, both copies of the CNTN6 gene, which were approximately 1 Mb in size, showed no any additional structural rearrangements.
- Добавил в систему: Мензоров Алексей Гавриилович