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The new strategy for studying drug delivery systems with prolonged release: seven-day in vitro antibacterial actionстатья
- Авторы: Skuredina A.A., Kopnova T.Yu, Tychinina A.S., Golyshev S.A., Le-Deygen I.M., Belogurova N.G., Kudryashova E.V.
- Журнал: Molecules
- Том: 27
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 1
- Последняя страница: 16
- Номер статьи: 8026
- DOI: 10.3390/molecules27228026
- Аннотация: The new method of antibacterial drug activity investigation in vitro is proposed as a powerful strategy for understanding, how carriers affect drug action during long periods (7 days). In this paper, we observed fluoroquinolone moxifloxacin (MF) antibacterial efficiency in non-covalent complexes with sulfobutyl ether derivative of β-cyclodextrin (SCD) and its polymer (SCDpol). We conducted in vitro studies on two Escherichia coli strains that differ in surface morphology. It was found that MF losses its antibacterial action after 3-4 days in a liquid media, whereas the inclusion of the drug in SCD leads to the increase of MF’s antibacterial activity up to 1.4 times within 1-5 days of the experiment. In the case of MF-SCDpol, we observed a 12-fold increase of MF’s action and the tendency to prolonged antibacterial activity. We visualized this phenomenon (the state of bacteria, cell’s membrane, and surface morphology) during MF and MF-carrier exposure by TEM. SCD and SCDpol do not change the drug’s mechanism of action. The particles’ adsorption on cells is the crucial factor for determining the observed effects. The proteinaceous fimbria on bacteria’s surface gives a 2-fold increase of the drug carrier’s adsorption, hence the strains with fimbria are more preferable for proposed treatment. Furthermore, the approach to visualize CD’s polymer adsorption on bacteria via TEM is suggested. We hope that the proposed comprehensive method will be useful for the studies of drug delivery systems to uncover long-term antibacterial action.
- Добавил в систему: Скуредина Анна Алексеевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Acceptance-Certificate-molecules-2020236.pdf | Acceptance-Certificate-molecules-2020236.pdf | 234,9 КБ | 15 ноября 2022 [anna.skuredina] | |
| 2. | molecules-27-08026.pdf | molecules-27-08026.pdf | 4,9 МБ | 18 ноября 2022 [anna.skuredina] |