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From DNA-protein interactions to the genetic circuit design using CRISPR-dCas systemsстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Shaytan A.K., Novikov R.V., Vinnikov R.S., Gribkova A.K., Glukhov G.S.
- Журнал: Frontiers in Molecular Biosciences
- Том: 9
- Год издания: 2022
- Издательство: Frontiers Media S.A.
- Местоположение издательства: Lausanne, Switzerland
- Первая страница: 1
- Последняя страница: 10
- Номер статьи: 1070526
- DOI: 10.3389/fmolb.2022.1070526
- Аннотация: In the last decade, the CRISPR-Cas technology has gained widespread popularity in different fields from genome editing and detecting specific DNA/RNA sequences to gene expression control. At the heart of this technology is the ability of CRISPR-Cas complexes to be programmed for targeting particular DNA loci, even when using catalytically inactive dCas- proteins. The repertoire of naturally derived and engineered dCas-proteins including fusion proteins presents a promising toolbox that can be used to construct functional synthetic genetic circuits. Rational genetic circuit design, apart from having practical relevance, is an important step towards a deeper quantitative understanding of the basic principles governing gene expression regulation and functioning of living organisms. In this minireview, we provide a succinct overview of the application of CRISPR-dCas-based systems in the emerging field of synthetic genetic circuit design. We discuss the diversity of dCas-based tools, their properties, and their application in different types of genetic circuits and outline challenges and further research directions in the field.
- Добавил в систему: Шайтан Алексей Константинович