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Marker Systems Based on MicroRNA Gene Methylation for the Diagnosis of Stage I-II Breast Cancerстатья
- Авторы: Braga EA, Filippova EA, Loginov VI, Pronina IV, Burdennyi AM, Kazubskaya TP, Fridman MV, Khodyrev DS, Kushlinskii NE
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 168
- Номер: 2
- Год издания: 2019
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 280
- Последняя страница: 284
- Аннотация: Groups of microRNA genes, methylation of which is associated with the initial (I-II) stages of breast cancer, are determined, and new markers and marker systems for the disease diagnosis were created on the basis of these data. A total of 14 genes in which methylation was associated with breast cancer were identified with the use of methyl-specific PCR on a representative sample of 70 tumor specimens. Analysis of 46 specimens from patients with clinical stages I and II detected 9 genes (MIR-124-1, MIR-124-3, MIR-125b-1, MIR-129-2, MIR-132, MIR-148a, MIR-193a, MIR-34b/c, and MIR-9-3), in which methylation was associated with the initial stages of the disease. Using ROC analysis, we formed two systems including 6 markers each and detecting breast cancer at stages I-II with high sensitivity (89 and 91%) and specificity (88%) at AUC=0.92-0.93. These sets were validated on the total sample of 70 specimens including all disease stages; they showed 93 and 94% sensitivities, 88% specificity, and AUC=0.95. Highly sensitive systems of markers, based on microRNA gene methylation, were created for the diagnosis of breast cancer at stages I-II.
- Добавил в систему: Казубская Татьяна Павловна