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Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysisстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Golovina A.Y., Dzama M.M., Petriukov K.S., Zatsepin T.S., Sergiev P.V., Bogdanov A.A., Dontsova O.A.
- Журнал: Nucleic Acids Research
- Год издания: 2014
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- DOI: 10.1093/nar/gkt1160
- Аннотация: Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m6A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m6A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
- Добавил в систему: Сергиев Петр Владимирович