|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Flow-Seq Method: Features and Application in Bacterial Translation Studiesстатья
Статья опубликована в журнале из списка Web of Science и/или Scopus
- Авторы: Komarova Ekaterina S., Dontsova Olga A., Pyshnyi Dmitry V., Kabilov Marsel R., Sergiev Petr V.
- Журнал: Acta Naturae (англоязычная версия)
- Том: 14
- Номер: 4
- Год издания: 2023
- Издательство: Park Media Ltd
- Местоположение издательства: Moscow, Russia (Federation)
- Первая страница: 20
- Последняя страница: 37
- DOI: 10.32607/actanaturae.11820
- Аннотация: The Flow-seq method is based on using reporter construct libraries, where a certain element regulating the gene expression of fluorescent reporter proteins is represented in many thousands of variants. Reporter construct libraries are introduced into cells, sorted according to their fluorescence level, and then subjected to next-generation sequencing. Therefore, it turns out to be possible to identify patterns that determine the expression efficiency, based on tens and hundreds of thousands of reporter constructs in one experiment. This method has become common in evaluating the efficiency of protein synthesis simultaneously by multiple mRNA variants. However, its potential is not confined to this area. In the presented review, a comparative analysis of the Flow-seq method and other alternative approaches used for translation efficiency evaluation of mRNA was carried out; the features of its application and the results obtained by Flow-seq were also considered.
- Добавил в систему: Комарова Екатерина Сергеевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|