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Catalytically synthesized Prussian Blue nanozymes as labels for electrochemical DNA/RNA sensorsстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Komkova Maria A., Shavokshina Vera A., Zarochintsev Alexander A., Melnik Denis M., Aparin Ilya O., Zatsepin Timofei S., Karyakin Arkady A.
- Журнал: Talanta
- Год издания: 2023
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 124337
- DOI: 10.1016/j.talanta.2023.124337
- Аннотация: We propose catalytically synthesized nanozymes based on Prussian Blue (PB) and azidomethyl-substituted poly (3,4-ethylenedioxythiophene) (azidomethyl-PEDOT) as novel electrocatalytic labels for DNA/RNA sensors. Catalytic approach allowed to synthesize highly redox and electrocatalytically active Prussian Blue nanoparticles functionalized with azide groups that enable ‘click’ conjugation with alkyne-modified oligonucleotides. Both competitive and sandwich-type schemes were realized. As the sensor response the direct (mediator-free) electrocatalytic current of H2O2 reduction can be measured, which is proportional to the concentration of the hybridized labeled sequences. The current of H2O2 electrocatalytic reduction is only 3–8 times increased in the presence of the freely diffusing mediator catechol, which indicates high efficiency of direct electrocatalysis with the elaborated labels. Electrocatalytic amplification of the signal allows robust detection of (63–70)-base target sequences with concentrations below 0.2 nM in blood serum within an hour. We believe, the use of advanced Prussian Blue based electrocatalytic labels sets new avenues for robust point-of-care DNA/RNA sensing.
- Добавил в систему: Комкова Мария Андреевна