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Block by extracellular Mg2+ of single human purinergic P2X4 receptor channels expressed in human embryonic kidney cellsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 апреля 2017 г.
- Авторы: Negulyaev Y.A., Markwardt F.
- Журнал: Neuroscience Letters
- Том: 279
- Номер: 3
- Год издания: 2000
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 165
- Последняя страница: 168
- DOI: 10.1016/s0304-3940(99)00976-3
- Аннотация: Single channel properties of human P2X(4) receptors expressed in human embryonic kidney cells have been investigated by outside-out mode patch clamp recordings. P2X(4) channel activity was characterized by very fast kinetics. The current-voltage relationship was strongly non-linear at potentials <-100 mV. A slope conductance of approximate to 9 pS was estimated at the approximately linear part of the current-voltage relation (>-100 mV). External Mg2+ reversibly decreased the amplitude of ATP-evoked single channel currents in a concentration-dependent man ner but independent of the membrane potential. Additionally, extracellular Mg2+ shortened the mean open time whereas the mean closed time was not affected. Thus, Mg2+ ions are proposed to inhibit the function of human P2X(4) receptors by means of an open-channel block with a Mg2+ binding site at the exterior surface of the pore. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
- Добавил в систему: Негуляев Юрий Алексеевич