SODIUM-SELECTIVE CHANNELS IN MEMBRANES OF RAT MACROPHAGESстатья
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Дата последнего поиска статьи во внешних источниках: 18 апреля 2017 г.
Аннотация:With the use of the patch-clamp technique, highly selective nonvoltage-gated sodium channels were found in the membrane of rat peritoneal macrophages. The inward single channel currents were measured in cell-attached and outside-out mode experiments at different holding membrane potentials within the range of -60 to +40 mV. The channels had a unitary conductance of 10.2 +/- 0.2 pS with 145 mM Naf in the external solution at 23-24 degrees C. The results of ion-substitution experiments confirmed that this novel type of cation channel in macrophages is characterized by high selectivity for Na+ over K+ (as for Cs+, NH4+, Ca2+, Ba2+) ions, whose conduction through these sodium-permeable channels was not measurable. Lithium is the only other ion that is transported by this pathway; the unitary conductance was equal to 3.9 +/- 0.2 pS in the Li+-containing external solution. Single channel currents and conductance were found to be linearly dependent on the external sodium concentration. Sodium channels in macrophage membrane patches were not blocked by tetrodotoxin(0.01-1 mu M). Single sodium currents were reversibly inhibited by the external application of amiloride (0.1-2 mM) and its derivative ethylisopropilamiloride (0.01-0.1 mM). The mechanism of channel block by amiloride and its analogue seems to be different.