Stress promotes RNA G-quadruplex folding in human cells

Авторы: Kharel Prakash, Fay Marta, Manasova Ekaterina V., Anderson Paul J., Kurkin Alexander V., Guo Junjie U., Ivanov Pavel
Журнал: Nature communications
Том: 14
Год издания: 2023
Издательство: Nature Pub. Group
Местоположение издательства: United Kingdom
Номер статьи: 205
DOI: 10.1038/s41467-023-35811-x
Аннотация: Guanine (G)-rich nucleic acids can fold into G-quadruplex (G4) structures under permissive conditions. Although many RNAs contain sequences that fold into RNA G4s (rG4s) in vitro, their folding and functions in vivo are not well understood. In this report, we showed that the folding of putative rG4s in human cells into rG4 structures is dynamically regulated under stress. By using high-throughput dimethylsulfate (DMS) probing, we identified hundreds of endogenous stress-induced rG4s, and validated them by using an rG4 pulldown approach. Our results demonstrate that stress-induced rG4s are enriched in mRNA 3′-untranslated regions and enhance mRNA stability. Furthermore, stress-induced rG4 folding is readily reversible upon stress removal. In summary, our study revealed the dynamic regulation of rG4 folding in human cells and suggested that widespread rG4 motifs may have a global regulatoryimpact on mRNA stability and cellular stress response.
Добавил в систему: Куркин Александр Витальевич

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