Complex Analysis of Single-Cell RNA Sequencing Data

Khozyainova, A.A.; Valyaeva, A.A.; Arbatsky, M.S.; Isaev, S.V.; Iamshchikov, P.S.; Volchkov, E.V.; Sabirov, M.S.; Zainullina, V.R.; Chechekhin, V.I.; Vorobev, R.S.; Menyailo, M.E.; Tyurin-Kuzmin, P.A.; Denisov, E.V.

Авторы: Khozyainova Anna A., Valyaeva Anna A., Arbatsky Mikhail S., Isaev Sergey V., Iamshchikov Pavel S., Volchkov Egor V., Sabirov Marat S., Zainullina Viktoria R., Chechekhin Vadim I., Vorobev Rostislav S., Menyailo Maxim E., Tyurin-Kuzmin Pyotr A., Denisov Evgeny V.
Журнал: Biochemistry (Moscow)
Том: 88
Номер: 2
Год издания: 2023
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 231
Последняя страница: 252
DOI: 10.1134/s0006297923020074
Аннотация: Single-cell RNA sequencing (scRNA-seq) is a revolutionary tool for studying the physiology of normal and pathologically altered tissues. This approach provides information about molecular features (gene expression, mutations, chromatin accessibility, etc.) of cells, opens up the possibility to analyze the trajectories/phylogeny of cell differentiation and cell–cell interactions, and helps in discovery of new cell types and previously unexplored processes. From a clinical point of view, scRNA-seq facilitates deeper and more detailed analysis of molecular mechanisms of diseases and serves as a basis for the development of new preventive, diagnostic, and therapeutic strategies. The review describes different approaches to the analysis of scRNA-seq data, discusses the advantages and disadvantages of bioinformatics tools, provides recommendations and examples of their successful use, and suggests potential directions for improvement. We also emphasize the need for creating new protocols, including multiomics ones, for the preparation of DNA/RNA libraries of single cells with the purpose of more complete understanding of individual cells.
Добавил в систему: Валяева Анна Александровна

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Complex Analysis of Single-Cell RNA Sequencing Dataстатья

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