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Bienzyme “nanozymes” struggling with oxidative stressтезисы доклада
- Авторы: Угланова С.В., Лопухов А.В., Зайцева Е.А., Кабанов А.В., Клячко Н.Л.
- Сборник: Сб. Тезисов докладов Шестого международного конгресса «Биотехнология. Современное состояние и перспективы развития»
- Том: 2
- Тезисы
- Год издания: 2011
- Место издания: ЗАО "Экспо-биохим-технологии" Москва
- Первая страница: 294
- Последняя страница: 295
- Аннотация: Method of enzyme encapsulation with block ionomers was developed for drug target delivery for post apoplectic period therapy, Alzheimer's disease, Parkinson’s disease, neurosis, vascular dementia, inflammation etc. Bienzyme systems generation considered to be perspective. In one particle, there are antioxidant enzymes (catalase, SOD, glutathione peroxidase) catalyzing sequential reactions of reactive oxigen species ROS elimination. A wide range of enzyme or bienzyme containing systems with block-ionomer was obtained: polymer shell composition, charge varied. Bionanosystems based on complexes of an enzyme (catalase, SOD, glutathione peroxidase) and a block ionomer with different length and branching of the charged chain termed “nanozymes”. [Brynskikh A., Zhao Y., Mosley R., Li S., Boska M. , Klyachko N., Kabanov A., Gendelman H., Batrakova E. Macrophage delivery of therapeutic nanozymes in a murine model of Parkinson’s disease.// Nanomedicine (Lond). 2010. 5(3). P. 379–396]. Polymer shell formation with protein core is based on the electrostatic interactions of oppositely charged groups of the components. For crosslinking, one can use well known crosslinking agents, the developed technique preserve system stability and enzyme activity after encapsulation. We demonstrate that enzyme-ionomer nanoparticles display better protease stability. Use of block ionomer opens possibilities for surface modification and supposed to be perspective for drug delivery through the BBB. Investigations were financially supported by Russian Ministry of Science and Education (grant №02.740.11.5231, grant №11.G34.31.0004).
- Добавил в систему: Зайцева Елена Анатольевна