Аннотация:The purpose of the current laboratory study was to study the effect of low-intensity energy of a neodymium laser with a wavelengthof 1064 nm and a short pulse (650 μs) on the wound process in mice. For the two experiments, we took 33 mice of the C57bl/6line and 50 mice of the FVB/d20nt line of their own breeding at the age of 12-20 days. The animals were divided into groups, thecontrol group was general, and other groups were formed on the basis of the laser exposure obtained. Mice were removed from theexperiment simultaneously by euthanasia (cervical dislocation), after which the material (back skin) was taken and the sectionswere stained with hematoxylin-eosin and sent for morphological analysis.After IHC analysis, it should be considered that the neodymium laser used in the presented laboratory study contributes to theacceleration of the onset of the proliferation phase. In the process of this study, it was revealed that migration of macrophages, mastcells and fibroblasts into the wound occurs during LLLT.During the main stage of the experiment, it was found that for the study of scar formation, the mouse is not the most optimalobject, since wounds in mice heal quickly enough without any effects, respectively, it is impossible to assess the effect of the laseron the entire wound process and, especially, the phase of scar remodeling. LLLT should be used from the moment of wounding andlaser treatment should be carried out for 48 hours, after which the animals should be removed from the experiment for histologicalexamination; and histological sections of all groups of animals (LLLT 2 and control group with excretion after 24 and 48 hours) should be followed and markers of angiogenesis should be determined, as well as markers of angiogenesis, as well as markers of angiogenesis. It is necessary to conduct a study with the participation of a biophysicist to study the interaction of neodymium laser energy with blood and plasma proteins.
