Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Shirshin, E.A.; Gurfinkel, Y.I.; Priezzhev, A.V.; Fadeev, V.V.; Lademann, J.; Darvin, M.E.

Авторы: Shirshin Evgeny A., Gurfinkel Yury I., Priezzhev Alexander V., Fadeev Victor V., Lademann Juergen, Darvin Maxim E.
Журнал: Scientific reports
Том: 7
Номер: 1171
Год издания: 2017
Издательство: Nature Publishing Group
Местоположение издательства: United Kingdom
DOI: 10.1038/s41598-017-01238-w
Аннотация: The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.
Добавил в систему: Ширшин Евгений Александрович

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Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localizationстатья

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