Abundance and Diversity of Microorganisms in Soils and Associated Substrates (Leaf Litter and “Suspended Soil”) in Some Nature Reserves of Vietnamстатья
Статья опубликована в журнале из списка Web of Science и/или Scopus
Аннотация:A comprehensive study of prokaryotic communities of zonal Ferralsols and intrazonal Fluvisols, aswell as associated leaf litter and “suspended” soil from epiphytic fern baskets was carried out. The maximumnumber of bacteria determined by the direct luminescence method (5.59 billion cells) was found in the samples of Fluvisols, while the lengths of fungal (2038 m/g) and actinomycete (1086 m/g) mycelia were the largestin the mountainous Ferralsols. Bacteria of the genus Streptomyces included in the saprotrophic bacterial complex of the studied substrates made a significant contribution to the destruction of plant material. The bacteria of the phyla Firmicutes (80%) and Proteobacteria (15%) predominated in the prokaryotic community ofthe zonal Ferralsols, while the phyla Proteobacteria (51%) and Actinobacteria (38%) dominated in the sample of the “suspended” soil. The phyla Chloroflexi, Acidobacteria, Bacteroidetes, and Cyanobacteria weresignificantly less represented. Against the background of significant differences between the studied prokaryotic communities at the level of genera, they were characterized by similar functional groups of microorganisms: xenobiotic decomposers, nitrogen cycle bacteria, extremophiles, as well as bacteria that inhibit thegrowth of micromycetes. The metabolically active part of the prokaryotic community represented by thephyla Proteobacteria, Actinobacteria, and Acidobacteria, was the highest in the “suspended” soil and theleast active in the leaf litter and A horizon of Ferralsols, which correlated with the high abundance of thesephyla and the significant taxonomic diversity of bacteria in this locus. Functional genes nifH and alkB encoding nitrogenase and alkane-hydroxylase, respectively, were detected in all studied substrates. The number ofcopies of functional genes was the highest in the “suspended” soil sample, which makes this locus promisingfor isolating strains with a high biotechnological potential.
