New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentrationстатья
Информация о цитировании статьи получена из
Web of Science ,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 13 июня 2017 г.
Авторы:
Duffy E. ,
Padovani R. ,
He X. ,
Gorkin R. ,
Vereshchagina E. ,
Ducrée J. ,
Nesterenko E. ,
Nesterenko P.N. ,
Brabazon D. ,
Paull B. ,
Vázquez M.
Журнал:
Analytical Methods
Том:
9
Номер:
13
Год издания:
2017
Издательство:
Royal Society of Chemistry
Местоположение издательства:
United Kingdom
Первая страница:
1998
Последняя страница:
2006
DOI:
10.1039/c7ay00127d
Аннотация:
New approaches for fabrication of centrifugal microfluidic platforms (μCDs) for sample micro-extraction and pre-concentration in bioanalytical and environmental applications are presented. The integration of both octadecylsilica (C18) micro-particulate and porous carbon monolithic stationary phases was demonstrated and on-disc extractions of analytes in samples of different nature were performed. A novel strategy based on the packing of micro-particulate stationary phases using porous organic polymer monoliths as column frits was demonstrated through the in situ photo-polymerisation of monolithic frits in a specific area of the micro-channel, thereby greatly facilitating stationary phase packing within μCD platforms. An enrichment factor of 3.7 was obtained for vitamin B12 following on-disc pre-concentration on the octadecylsilica columns. UV-Vis absorbance measurements were also performed in the outlet reservoir permitting quasi-on-line analysis of the small volume fractions collected after extraction, with limits of detection (LODs) found for vitamin B12 (LOD = 43 μM) being rather similar to those found with a commercially available spectrophotometer (LOD = 37 μM). Furthermore, the first integration of carbon monoliths within microfluidic channels is reported. Carbon monoliths were fabricated as rods and cut into discs for their integration within the microfluidic network, offering a highly porous bimodal structure with low flow-through back-pressures, excellent chemical stability as well as adequate mechanical stability. The carbon monolith-based μCD platform was evaluated as a rapid semi-automated pre-concentration approach suitable for in-field use prior to in-lab HPLC quantitation of pollutants at low concentration levels. Calculated mean recoveries for phenol from tap water spiked-samples by using this on-disc pre-concentration method were 68 ± 4% (n = 4, RSD = 5%). © The Royal Society of Chemistry.
Добавил в систему:
Нестеренко Павел Николаевич