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Alteration of Enzyme-substrate Interactions by Ultra-high Dilutions of the Substrateстатья
- Авторы: Lomakina G.Yu, Ugarova N.N.
- Журнал: Biophysics
- Том: 68
- Номер: 2
- Год издания: 2023
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 167
- Последняя страница: 175
- Аннотация: In this work, we examined the effect of a panel of ultra-high dilutions (UHDs) of luciferin ranging from 1010 to 10102 on luciferin-luciferase reaction of fireflies. Luciferin substrate concentration varied from 0.025 to 2.5 nM and concentration of ATP was constant. Comparison of the bioluminescence intensity after the addition of various UHDs of luciferin or UHDs of water to the bioluminescent system showed some multidirectional and significant differences. The number of individual UHDs from the studied panel for which significant differences were detected increased sharply with the reduction in the substrate concentration to 0.025 nM. Similar comparison of the effects of UHDs of a nonspecific control imidazole and UHDs of water on the signal intensity did not uncover any differences. Thus, it may be concluded that the presence of UHDs of luciferin specifically inhibits the luciferin-luciferase reaction
- Добавил в систему: Угарова Наталья Николаевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | текст статьи | Biofizika-2023-angl.pdf | 566,4 КБ | 21 июля 2023 [UgarovaNN] |
| 2. | заклдчение об открытой публикации | Biophizika2023-Zakl.pdf | 3,1 МБ | 21 июля 2023 [UgarovaNN] |