Inhibition of Oxidative Hemolysis in Erythrocytes by Mitochondria-Targeted Antioxidants of SkQ Seriesстатья
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Аннотация:In the present work we studied the effect of antioxidants of the SkQ1 family 10(6′plastoquinonyl)decyltri
phenylphosphonium) on the oxidative hemolysis of erythrocytes induced by a lipophilic free radical initiator 2,2′azo
bis(2,4dimethylvaleronitrile) (AMVN) and a watersoluble free radical initiator 2,2′azobis(2methylpropionamidine)
dihydrochloride (AAPH). SkQ1 was found to protect erythrocytes from hemolysis, 2 μM being the optimal concentration.
Both the oxidized and reduced SkQ1 forms exhibited protective properties. Both forms of SkQ1 also inhibited lipid perox
idation in erythrocytes induced by the lipophilic free radical initiator AMVN as detected by accumulation of malondialde
hyde. However, in the case of induction of erythrocyte oxidation by AAPH, the accumulation of malondialdehyde was not
inhibited by SkQ1. In the case of AAPHinduced hemolysis, the rhodaminecontaining analog SkQR1 exerted a compara
ble protective effect at the concentration of 0.2 μM. At higher SkQ1 and SkQR1 concentrations, the protective effect was
smaller, which was attributed to the ability of these compounds to facilitate hemolysis in the absence of oxidative stress. We
found that plastoquinone in the oxidized form of SkQ1 could be reduced by erythrocytes, which apparently accounted for
its protective action. Thus, the protective effect of SkQ in erythrocytes, which lack mitochondria, proceeded at concentra
tions that are two to three orders of magnitude higher than those that were active in isolated mitochondria.