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Stabilization of Erwinia carotovora and Rhodospirillum rubrum L-Asparaginases in Complexes with Polycationsстатья
- Авторы: Dobryakova N.D., Kudryashova E.V.
- Журнал: Applied Biochemistry and Microbiology
- Том: 59
- Номер: 9
- Год издания: 2023
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 34
- Последняя страница: 43
- DOI: 10.1134/S000368382309003X
- Аннотация: The effect of chitosan copolymers and polyethyleneimine (PEI) on the specific activity of L-asparaginases of Erwinia carotovora (EwA) and Rhodospirillum rubrum (RrA) at different pH values, as well as theinfluence of polycations on the mechanisms of thermal inactivation and trypsinolysis of the enzymes, hasbeen studied. Using circular dichroism spectroscopy, the catalytic activities of polyelectrolyte complexes(PEC) were determined, as well as changes in the structure of native enzymes before and after incubation atelevated temperatures. For most PECs, the pH optimum was shifted by 0.5 units towards more acidic valuesdue to the polycationic nature of the polyelectrolytes. The inactivation of EwA and its complexes was foundto be associated predominantly with aggregation, while RrA underwent denaturation without aggregation. Astabilizing effect of complex formation was observed: the EwA aggregation was slowed as a result of the formation of PECs with chitosan and its copolymers. The effect of chitosan in stabilizing EwA upon heating washigher than that of RrA. The PEC of RrA with PEI turned out to be optimal: the first order thermal inactivation constant was decreased by 2.2 times compared to the native enzyme and the inactivation constant duringtrypsinolysis was reduced two-fold. The chitosan-PEG complexation made it possible to maintain or evenincrease the high level of catalytic activity of the enzymes with simultaneous enhancement of their thermalstability.
- Добавил в систему: Добрякова Наталья Владимировна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | AppBio2309003DobryakovaKOR.pdf | 824,9 КБ | 7 сентября 2023 [Natadobryak] |