An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser

Assalauova, D.; Kim, Y.Y.; Bobkov, S.; Khubbutdinov, R.; Rose, M.; Alvarez, R.; Andreasson, J.; Balaur, E.; Contreras, A.; DeMirci, H.; Gelisio, L.; Hajdu, J.; Hunter, M.S.; Kurta, R.P.; Li Haoyuan; McFadden, M.; Nazari, R.; Schwander, P.; Teslyuk, A.; Walter, P.; Xavier, P.L.; Yoon, C.H.; Zaare, S.; Ilyin, V.A.; Kirian, R.A.; Hogue, B.G.; Aquila, A.; Vartanyants, I.A.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.

Авторы: Assalauova Dameli, Kim Young Yong, Bobkov Sergey, Khubbutdinov Ruslan, Rose Max, Alvarez Roberto, Andreasson Jakob, Balaur Eugeniu, Contreras Alice, DeMirci Hasan, Gelisio Luca, Hajdu Janos, Hunter Mark S., Kurta Ruslan P., Li Haoyuan, McFadden Matthew, Nazari Reza, Schwander Peter, Teslyuk Anton, Walter Peter, Xavier P.Lourdu, Yoon Chun Hong, Zaare Sahba, Ilyin Viacheslav A., Kirian Richard A., Hogue Brenda G., Aquila Andrew, Vartanyants Ivan A.
Журнал: International Union of Crystallography Journal
Том: 7
Номер: 6
Год издания: 2020
Первая страница: 1102
Последняя страница: 1113
DOI: 10.1107/s2052252520012798
Аннотация: An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent Light Source as part of the SPI initiative. Existing methods were modified to cope with the shortcomings of the experimental data: inaccessibility of information from half of the detector and a small fraction of single hits. The general SPI analysis workflow was upgraded with the expectation-maximization based classification of diffraction patterns and mode decomposition on the final virus-structure determination step. The presented processing pipeline allowed us to determine the 3D structure of bacteriophage PR772 without symmetry constraints with a spatial resolution of 6.9 nm. The obtained resolution was limited by the scattering intensity during the experiment and the relatively small number of single hits.
Добавил в систему: Ильин Вячеслав Анатольевич

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An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laserстатья