Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate

Kolmogorov, V.; Erofeev, A.; Vaneev, A.; Gorbacheva, L.; Kolesov, D.; Klyachko, N.; Korchev, Y.; Gorelkin, P.

Авторы: Kolmogorov Vasilii, Erofeev Alexander, Vaneev Alexander, Gorbacheva Lyubov, Kolesov Dmitry, Klyachko Natalia, Korchev Yuri, Gorelkin Petr
Журнал: Cells
Том: 12
Номер: 20
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1
Последняя страница: 7
Номер статьи: 2428
DOI: 10.3390/cells12202428
Аннотация: Mechanical properties of neuronal cells have a key role for growth, generation of traction forces, adhesion, migration, etc. Mechanical properties are regulated by chemical signaling, neurotransmitters, and neuronal ion exchange. Disturbance of chemical signaling is accompanied by several diseases such as ischemia, trauma, and neurodegenerative diseases. It is known that the disturbance of chemical signaling, like that caused by glutamate excitotoxicity, leads to the structural reorganization of the cytoskeleton of neuronal cells and the deviation of native mechanical properties. Thus, to investigate the mechanical properties of living neuronal cells in the presence of glutamate, it is crucial to use noncontact and low-stress methods, which are the advantages of scanning ion-conductance microscopy (SICM). Moreover, a nanopipette may be used for the local delivery of small molecules as well as for a probe. In this work, SICM was used as an advanced technique for the simultaneous local delivery of glutamate and investigation of living neuronal cell morphology and mechanical behavior caused by an excitotoxic effect of glutamate
Добавил в систему: Горбачева Любовь Руфэльевна

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