The integrated approach to the investigation of soluble multisubunit protein complexes in cyanobacterial cellsстатья
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Дата последнего поиска статьи во внешних источниках: 13 июня 2017 г.
Аннотация:Functional proteomics is currently one of the most important tools used for the studying of complex intracellular processes involving a variety of proteins that interact with each other with different probability. Many of them, fulfilling their functions within the complex network of interactions, affect functions of other proteins. Despite the fact that many of the components of these “protein networks” could be previously described individually, it did not give complete details about nature and components of the proposed interactions under natural conditions. The article describes the methods (native gel electrophoresis, size exclusion chromatography, and immuneprecipitation), the most effective in the study of protein interactions and optimized for the study of soluble multisubunit protein complexes in the cells of photosynthetic bacteria using a unicellular cyanobacterium Synechococcus elongates PCC 7942.