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Anticancer and antibacterial potential of methotrexate loaded iron nanoparticles produced by green synthesis methodстатья
- Авторы: Batsalova Tsvetelina, Moten Dzhemal, Voronova Anastasiia, Dzhambazov Balik, Vasil'kov Alexander
- Журнал: International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
- Том: 23
- Номер: 6.1
- Год издания: 2023
- DOI: 10.5593/sgem2023/6.1/s24.01
- Аннотация: An environmentally safe method – metal-vapor synthesis (MVS), was applied to produce iron nanoparticles (Fe NPs), which were conjugated with the drug methotrexate (MTX). The physicochemical properties and biological activity of the generated Fe NPs-MTX nanomaterials were analyzed. Transmission electron microscopy observations indicated that iron nanoparticles are stabilized in the form of aggregates with a size of 100 nm. The structure of these aggregates is similar to a “bunch of grapes,” which consists of metal particles about 5 – 12 nm in size. X-ray photoelectron spectroscopy evaluations showed that Fe both in the black and the composite with methotrexate were Fe 3+ state, and the Fe 2+ state is also present in a small amount. Biological activity of Fe NPs-MTX conjugates against bacterial and human cells was assessed. Agar disc diffusion assays with Gram-positive and Gram-negative bacteria were performed in combination with minimal inhibiting concentration analyses and longitudinal evaluations of bacterial growth inhibition. The cytotoxicity and anticancer potential of Fe NPs-MTX was determined by two types of in vitro assays using three cancer cell lines and normal fibroblasts. Our results demonstrated inhibitory effects of the nanoconjugates against both Gram-positive and Gram-negative bacteria that were superior compared to the activity of unloaded nanoparticles and methotrexate solution. Analyses with human cell lines showed time- and concentration-dependent cytotoxicity of Fe NPs-MTX against cancer cells and low inhibitory activity towards normal fibroblasts proving the anticancer potential of the nanoconjugates. In addition, colon adenocarcinoma-specific effect was observed based on the detection of highest inhibitory effects of MTX-Fe NPs against HT29 cell line. In vitro assays initially demonstrated lysosome-directed toxicity which after a longer exposition period (72-120h) was superseded by pronounced inhibition of cellular metabolic activity. These data indicate a wide biological functionality spectrum of MVS produced Fe NPs loaded with MTX and their potential for biomedical application.
- Добавил в систему: Воронова Анастасия Андреевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
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| 1. | Ekspertnoe_zaklyuchenie.pdf | Ekspertnoe_zaklyuchenie.pdf | 2,1 МБ | 14 ноября 2023 [voronnasvaa] |