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The Characteristics of Bacillus Cereus Complex Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks during Climate Changeстатья
- Авторы: Goncharova Y.O., Evseeva V.V., Mironova R.I., Khlopova K.V., Bogun A.G., Sizova A.A., Solomentsev V.I., Bahtejeva I.V., Titareva G.M., Kravchenko T.B., Brushkov A.V.
- Журнал: Applied Biochemistry and Microbiology
- Том: 59
- Номер: 6
- Год издания: 2023
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 808
- Последняя страница: 817
- Аннотация: Three strains of the anthrax pathogen were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the species of the Bacillus cereus complex. The strains were genetically characterized by RAPD genotyping. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differentiate the studied strains from the B. anthracis species and to show their intraspecific genetic differences and the degree of relationship. Whole genome sequencing was carried out, based on the data of which MLST genotyping was carried out, which revealed two known sequence types and one new one that is described for the first time in this work. The results we obtained are of practical importance and are extremely interesting from the point of view of the evolution and phylogeography of the B. cereus complex group, since the fact that strains were isolated from permafrost suggests that their age may be much older than expected. Keywords: Bacillus cereus complex