Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Method

Sobolev, P.D.; Burnakova, N.A.; Beloborodova, N.V.; Revelsky, A.I.; Pautova, A.K.

Авторы: Sobolev Pavel D., Burnakova Natalia A., Beloborodova Natalia V., Revelsky Alexander I., Pautova Alisa K.
Журнал: Metabolites
Том: 13
Номер: 11
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1
Последняя страница: 15
Номер статьи: 1128
DOI: 10.3390/metabo13111128
Аннотация: The profile of and dynamic concentration changes in tyrosine, phenylalanine, and tryptophan metabolites in blood are of great interest since they could be considered potential biomarkers ofdifferent disorders. Some aromatic metabolites, such as 4-hydroxyphenyllactic, 4-hydroxyphenylacetic,phenyllactic, and 4-hydroxybenzoic acids have previously demonstrated their diagnostic significancein critically ill patients and patients with post-COVID-19 syndrome. In this study, a sensitive method,including serum protein precipitation with methanol and ultra-high-pressure liquid chromatographytandem mass spectrometry (UPLC-MS/MS) detection, was developed and validated for six phenyland five indole-containing acids in human serum. The liquid–liquid extraction was also examined,but it demonstrated unsatisfactory results based on analyte recoveries and the matrix effect. However,the recoveries for all analytes reached 100% and matrix effects were not observed using proteinprecipitation. This made it possible to use deionized water as a blank matrix. The lower limitsof quantitation (LLOQs) were from 0.02 to 0.25 µmol/L. The validated method was used for theanalysis of serum samples of healthy volunteers (n = 48) to reveal the reference values of the targetanalytes. The concentrations of the most clinically significant metabolite 4-hydroxyphenyllactic acid,which were revealed using UPLC-MS/MS and a previously developed gas chromatography-massspectrometry method, were completely comparable. The proposed UPLC-MS/MS protocol can beused in the routine clinical practice of medical centers.
Добавил в систему: Ревельский Александр Игоревич

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Analysis of 4-Hydroxyphenyllactic Acid and Other Diagnostically Important Metabolites of α-Amino Acids in Human Blood Serum Using a Validated and Sensitive Ultra-High-Pressure Liquid Chromatography-Tandem Mass Spectrometry Methodстатья

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