Knockout of BAX, BAK1 Genes and Overexpression of BCL2, BECN1 Genes Increase Lifespan and the Maximum Density of a  CHO-S Cell Culture

Авторы: Kovnir S.V., Dayanova L.K., Gaiamova E.A., Dybovsky L.N., Vorobiev I.I., Orlova N.A.
Журнал: Applied Biochemistry and Microbiology
Том: 59
Номер: 8
Год издания: 2023
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 1047
Последняя страница: 1052
Аннотация: Two genome editions have resulted in the CHO cell line 4BGD with homozygous knockouts of the BAK1, BAX, DHFR and GLUL genes, and overexpression of the BCL2 and BECN1 genes. This line is capable of long-term growth without changing the culture medium and exhibits a fivefold increase in the integral cell density compared to the parental cell line under the same cultivation regime. Overexpression of the BCL2, BECN1 gene pair led to a significant increase in cell density on days 6‒10 of cultivation compared to the cell line with a knockout of the BAK1, BAX, DHFR and GLUL genes, while the integral viable cell density increases by 34%.
Добавил в систему: Воробьев Иван Иванович

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