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Method for Collection of Tear Fluid for Evaluation Its Antioxidant Propertiesстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.
- Авторы: Tyulina V.V., Senin I.I.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 175
- Номер: 4
- Год издания: 2023
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 492
- Последняя страница: 496
- DOI: 10.1007/s10517-023-05893-0
- Аннотация: A technique of collection of the tear fluid with Schirmer strips for evaluation of the activity of the main antioxidant defense enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in the tear fluid was proposed. The degree of extraction of the studied enzymes from the Schirmer strip is >85%. Cytometry showed that conjunctival and corneal cells can be transferred to the Schirmer strips during tear collection, which leads to sample contamination with intracellular fractions of the antioxidant enzymes. The approach proposed by us allows avoiding this contamination during tear fluid sampling. This technique makes it possible to increase the accuracy of determining the activity of antioxidant protection enzymes in the tear fluid and can be used for diagnostics of ocular surface pathologies in clinical practice.Keywords: Shirmer strips; catalase; glutathione peroxidase; superoxide dismutase; tear fluid.
- Добавил в систему: Тюлина Вероника Валерьевна