The chloroplast genome of black currant (DNA.) cultivar 'Kipiana': genome structure and chloroplast Simple Sequence RepeatsстатьяИсследовательская статья
Статья опубликована в журнале из списка Web of Science и/или Scopus
Аннотация:The study has been conducted in year 2022-2023 at Russian Research Instituteof Fruit Crop Breeding (VNIISPK), 302530 Orel, Russia with help of computationalresources provided by the Belozersky Institute of Physico-Chemical Biology, LomonosovMoscow State University, Moscow, Russia. Ribes nigrum, commonly known as blackcurrant, is a perennial deciduous shrub having an economic importance. Though ofeconomical importance of Ribes nigrum its complete chloroplast genome sequences isnot published yet. Here, the complete chloroplast genome of black currant cultivar ‘Kipiana’was sequenced using Illumina sequencing technology and de novo assembled for thefirst time. Simple sequence repeats were detected and located in the chloroplast genome.The plastome is 157,450 bp in length, with a GC content of 38.1%. The chloroplastgenome is features a typical quadripartite structure, consisting of a pair of invertedrepeat regions of 26,007 bp for each repeat unit, separated by a large single copy regionof 87,086 bp, and a small single copy region of 18,350 bp. A total of 133 genes wereannotated in the plastome, including 85 protein coding genes, 37 tRNA genes, 8 rRNAgenes and 3 pseudogenes (both copies of ycf15 and one copy of ycf1 gene). Totally 66simple sequence repeats (SSRs) were identified within the assembled Ribes nigrum plastidgenome. Thus, our study provides genomic resources and valuable reference for markerdevelopment and phylogenomics in Ribes.
