Extensive long-range polycomb interactions and weak compartmentalization are hallmarks of human neuronal 3D genome

Pletenev, I.A.; Bazarevich, M.; Zagirova, D.R.; Kononkova, A.D.; Cherkasov, A.V.; Efimova, O.I.; Tiukacheva, E.A.; Morozov, K.V.; Ulianov, K.A.; Komkov, D.; Tvorogova, A.V.; Golimbet, V.E.; Kondratyev, N.V.; Razin, S.V.; Khaitovich, P.; Ulianov, S.V.; Khrameeva, E.E.

Авторы: Pletenev Ilya A., Bazarevich Maria, Zagirova Diana R., Kononkova Anna D., Cherkasov Alexander V., Efimova Olga I., Tiukacheva Eugenia A., Morozov Kirill V., Ulianov Kirill A., Komkov Dmitriy, Tvorogova Anna V., Golimbet Vera E., Kondratyev Nikolay V., Razin Sergey V., Khaitovich Philipp, Ulianov Sergey V., Khrameeva Ekaterina E.
Журнал: Nucleic Acids Research
Год издания: 2024
Издательство: Oxford University Press
Местоположение издательства: United Kingdom
Первая страница: 1
Последняя страница: 19
DOI: 10.1093/nar/gkae271
Аннотация: Chromatin architecture regulates gene expression and shapes cellular identity, particularly in neuronal cells. Specifically, polycomb group (PcG)proteins enable establishment and maintenance of neuronal cell type by reorganizing chromatin into repressive domains that limit the expressionof fate-determining genes and sustain distinct gene expression patterns in neurons. Here, we map the 3D genome architecture in neuronal andnon-neuronal cells isolated from the Wernicke’s area of four human brains and comprehensively analyze neuron-specific aspects of chromatinorganization. We find that genome segregation into active and inactive compartments is greatly reduced in neurons compared to other braincells. Furthermore, neuronal Hi-C maps reveal strong long-range interactions, forming a specific network of PcG-mediated contacts in neuronsthat is nearly absent in other brain cells. These interacting loci contain developmental transcription factors with repressed expression in neuronsand other mature brain cells. But only in neurons, they are rich in bivalent promoters occupied by H3K4me3 histone modification together withH3K27me3, which points to a possible functional role of PcG contacts in neurons. Importantly, other layers of chromatin organization also exhibita distinct structure in neurons, characterized by an increase in short-range interactions and a decrease in long-range ones.
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Extensive long-range polycomb interactions and weak compartmentalization are hallmarks of human neuronal 3D genomeстатья Исследовательская статья

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