Femtosecond optical studies of the primary charge separation reactions in far-red photosystem II from Synechococcus sp. PCC 7335

Cherepanov, D.A.; Kurashov, V.; Gostev, F.E.; Shelaev, I.V.; Zabelin, A.A.; Shen, G.; Mamedov, M.D.; Aybush, A.; Shkuropatov, A.Y.; Nadtochenko, V.A.; Bryant, D.A.; Golbeck, J.H.; Semenov, A.Y.

Авторы: Cherepanov Dmitry A., Kurashov Vasily, Gostev Fedor E., Shelaev Ivan V., Zabelin Alexey A., Shen Gaozhong, Mamedov Mahir D., Aybush Arseny, Shkuropatov Anatoly Ya, Nadtochenko Victor A., Bryant Donald A., Golbeck John H., Semenov Alexey Yu
Журнал: Biochimica et Biophysica Acta - Bioenergetics
Том: 1865
Номер: 3
Год издания: 2024
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 149044
DOI: 10.1016/j.bbabio.2024.149044
Аннотация: Primary processes of light energy conversion by Photosystem II (PSII) were studied using femtosecond broadband pump-probe absorption difference spectroscopy. Transient absorption changes of core complexes isolated from the cyanobacterium Synechococcus sp. PCC 7335 grown under far-red light (FRL-PSII) were compared with the canonical Chl a containing spinach PSII core complexes upon excitation into the red edge of the Qy band. Absorption changes of FRL-PSII were monitored at 278 K in the 400–800 nm spectral range on a timescale of 0.1–500 ps upon selective excitation at 740 nm of four chlorophyll (Chl) f molecules in the light harvesting antenna, or of one Chl d molecule at the ChlD1 position in the reaction center (RC) upon pumping at 710 nm. Numerical analysis of absorption changes and assessment of the energy levels of the presumed ion-radical states made it possible to identify PD1 + ChlD1 􀀀 as the predominant primary charge-separated radical pair, the formation of which upon selective excitation of Chl d has an apparent time of ~1.6 ps. Electron transfer to the secondary acceptor pheophytin PheoD1 has an apparent time of ~7 ps with a variety of excitation wavelengths. The energy redistribution between Chl a and Chl f in the antenna occurs within 1 ps, whereas the energy migration from Chl f to the RC occurs mostly with lifetimes of 60 and 400 ps. Potentiometric analysis suggests that in canonical PSII, PD1 + ChlD1 􀀀 can be partially formed from the excited (PD1ChlD1)* state.
Добавил в систему: Семенов Алексей Юрьевич

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Femtosecond optical studies of the primary charge separation reactions in far-red photosystem II from Synechococcus sp. PCC 7335статья Исследовательская статья

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