Fluorescence saturation imaging microscopy: molecular fingerprinting with a standard confocal microscopeстатья
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Дата последнего поиска статьи во внешних источниках: 26 июня 2024 г.
Аннотация:Molecular specificity in fluorescence imaging of cells and tissues can be increasedby measuring parameters other than intensity. For instance, fluorescence lifetime imagingbecame a widespread modality for biomedical optics. Previously, we suggested using thefluorescence saturation effect at pulsed laser excitation to map the absorption cross-section asan additional molecular contrast in two-photon microscopy [Opt. Lett. 47, 4455 (2022)].Here, it is shown that, somewhat counterintuitive, fluorescence saturation can be observedunder cw excitation in a standard confocal microscopy setup. Mapping the fluorescencesaturation parameter allows obtaining additional information about the fluorophores in thesystem, as demonstrated by the example of peptide hydrogel, stained cells and unstainedthyroid gland. The suggested technique does not require additional equipment and can beimplemented on confocal systems as is.