Pilot study on boiling histotripsy treatment of human leiomyoma ex vivo

Ponomarchuk, E.; Tsysar, S.; Kvashennikova, A.; Chupova, D.; Pestova, P.; Danilova, N.; Malkov, P.; Buravkov, S.; Khokhlova, V.

Авторы: Ponomarchuk Ekaterina, Tsysar Sergey, Kvashennikova Anastasia, Chupova Daria, Pestova Polina, Danilova Natalia, Malkov Pavel, Buravkov Sergey, Khokhlova Vera
Журнал: Ultrasound in Medicine and Biology
Том: 50
Номер: 8
Год издания: 2024
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 1255
Последняя страница: 1261
DOI: 10.1016/j.ultrasmedbio.2024.05.002
Аннотация: Objective: As an alternative to surgical excision and magnetic resonance-guided thermal high-intensity focused ultrasound ablation of uterine leiomyoma, this work was aimed at pilot feasibility demonstration of use of ultrasound-guided boiling histotripsy for non-invasive non-thermal fractionation of human uterine leiomyoma ex vivo. Methods: A custom-made sector ultrasound transducer of 1.5-MHz operating frequency and nominal f-number F# = 0.75 was used to produce a volumetric lesion (two layers of 5 × 5 foci with a 1 mm step) in surgically resected human leiomyoma ex vivo. A sequence of 10 ms pulses (P+/P–/As = 157/–25/170 MPa in situ) with 1% duty cycle was delivered N = 30 times per focus under B-mode guidance. The treatment outcome was evaluated via B-mode imaging and histologically with hematoxylin and eosin and Masson's trichrome staining. Results: The treatment was successfully performed in less than 30 min and resulted in formation of a rectangular lesion visualized on B-mode images during the sonication as an echogenic region, which sustained for about 10 min post-treatment. Histology revealed loss of cellular structure, necrotic debris and globules of degenerated collagen in the target volume surrounded by injured smooth muscle cells. Conclusion: The pilot experiment described here indicates that boiling histotripsy is feasible for non-invasive mechanical disintegration of human uterine leiomyoma ex vivo under B-mode guidance, encouraging further investigation and optimization of this potential clinical application of boiling histotripsy.
Добавил в систему: Пономарчук Екатерина Максимовна

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Pilot study on boiling histotripsy treatment of human leiomyoma ex vivoстатья

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