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The distribution of different types of rotary ATPases among prokaryotesстатья Тезисы
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Litvin Anna, Lapashina Anna, Gelfand Mikhail, Feniouk Boris
- Журнал: Biochimica et Biophysica Acta - Bioenergetics
- Том: 1865
- Год издания: 2024
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Номер статьи: 149220
- DOI: 10.1016/j.bbabio.2024.149220
- Аннотация: Rotary ATPase is a multisubunit membrane enzyme that catalyses ATP synthesis using the transmembrane potential of protons or sodium ions. In some prokaryotes the enzyme catalyses the reverse reaction and generates transmembrane ion potential, working as an ATP-driven ion pump. A-type ATPases are present in archaea and bacteria, F-type enzymes are found almost exclusively in bacteria. N-type ATPases, similar to the F-ATPases, are presumably ATP-dependent ion pumps in the cell. Most prokaryotes use proton potential for ATP synthesis, sodium bioenergetics is rare and considered to be more ancient.The aim of the work was to identify and describe the prevalence of different types of rotary ATPases among prokaryotes. The GTDB v202 genomic database (45555 bacterial and 2339 archaeal genomes) was used in the work. Genes of ATPases were detected using hidden Markov models, grouped into colocalized clusters of subunit genes, and then into enzymes. The ion specificity was determined by the presence of a specific sodium-binding motif in the membrane c/K subunit. In total 37203 F-ATPases were identified, found in bacteria, most have proton specificity (35105 proton and 2078 sodium); 1774 NATPases, found mainly in bacteria, proton (879) and sodium (895) are equally common; 8783 A-ATPases, found both in archaea and bacteria, sodium (4755) enzymes found as often as proton (3981). Wedid not expect many prokaryotes not to have rotary ATPases. Nevertheless, the number of genomes with no detected genes of rotary ATPases turned out to be significant (3596). In some cases the absence of ATPase is due to genome incompleteness, however, for many organisms, the reason is probably a reduction in metabolism associated with an endosymbiotic or parasitic lifestyle. Organisms with several ATPases are of interest. N-type ion pumps are found in combination with F-ATPase in 1341 bacteria, or in combination with A-ATPase in 60 bacteria and 37 archaea. All three types of enzyme can be found in 142 bacteria. 4522 species (mostly bacteria) have several F- or A-type ATPases (from two to four) with different or identical ion specificity. Several groups with suspected sodium bioenergetics have been found — within phyla Firmicutes, Firmicutes_A/D/E/F/G/H, Patescibacteria, Chlamydiota, Verrucomicrobiota, Spirochaetota, Bipolaricauolata, Thermotogota, Fusobacteriota.
- Добавил в систему: Лапашина Анна Сергеевна