Disruption ofPoly(ADP-ribosyl)ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymes

Kalinina, N.O.; Spechenkova, N.; Ilina, I.; Samarskaya, V.O.; Bagdasarova, P.; Zavriev, S.K.; Love, A.J.; Taliansky, M.

Авторы: Kalinina Natalia O., Spechenkova Nadezhda, Ilina Irina, Samarskaya Viktoriya O., Bagdasarova Polina, Zavriev Sergey K., Love Andrew J., Taliansky Michael
Журнал: International Journal of Molecular Sciences
Том: 25
Год издания: 2024
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 9367
DOI: 10.3390/ijms25179367
Аннотация: ADP-ribosylation (ADPRylation) is a mechanism which post-translationally modifies pro-teins in eukaryotes in order to regulate a broad range of biological processes including programmedcell death, cell signaling, DNA repair, and responses to biotic and abiotic stresses. Poly(ADP-ribosyl)polymerases (PARPs) play a key role in the process of ADPRylation, which modifies target proteinsby attaching ADP-ribose molecules. Here, we investigated whether and how PARP1 and PARylationmodulate responses of Nicotiana benthamiana plants to methyl viologen (MV)-induced oxidative stress.It was found that the burst of reactive oxygen species (ROS), cell death, and loss of tissue viabilityinvoked by MV in N. benthamiana leaves was significantly delayed by both the RNA silencing ofthe PARP1 gene and by applying the pharmacological inhibitor 3-aminobenzamide (3AB) to inhibitPARylation activity. This in turn reduced the accumulation of PARylated proteins and significantlyincreased the gene expression of major ROS scavenging enzymes including SOD (NbMnSOD; mito-chondrial manganese SOD), CAT (NbCAT2), GR (NbGR), and APX (NbAPX5), and inhibited celldeath. This mechanism may be part of a broader network that regulates plant sensitivity to oxidativestress through various genetically programmed pathways.
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Disruption ofPoly(ADP-ribosyl)ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymesстатья

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