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Dual-stimuli-sensitive microgels as a tool for stimulated sponge-like adsorption of biomaterials for biosensor applicationsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 ноября 2014 г.
- Авторы: Sigolaeva L.V., Gladyr S.Yu, Gelissen A.P.H, Mergel O., Pergushov D.V., Kurochkin I.N., Plamper F.A., Richtering W.
- Журнал: Biomacromolecules
- Том: 15
- Номер: 10
- Год издания: 2014
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 3735
- Последняя страница: 3745
- DOI: 10.1021/bm5010349
- Аннотация: This work examines the fabrication regime and the properties of microgel and microgel/enzyme thin films adsorbed onto conductive substrates (graphite or gold). The films were formed via two sequential steps: the adsorption of a temperature- and pH-sensitive microgel synthesized by precipitation copolymerization of N-isopropylacrylamide (NIPAM) and 3-(N,N-dimethylamino)propylmethacrylamide (DMAPMA) (poly(NIPAM-co-DMAPMA) at the pH-condition corresponding to its noncharged state (first step of adsorption), followed by the enzyme, tyrosinase, adsorption at the pH-condition when the microgel and the enzyme are oppositely charged (second step of adsorption). The stimuli-sensitive properties of poly(NIPAM-co-DMAPMA) microgel were characterized by potentiometric titration and dynamic light scattering (DLS) in solution as well as by atomic force microscopy (AFM) and quartz crystal microbalance with dissipation monitoring (QCM-D) at solid interface. Enhanced deposition of poly(NIPAM-co-DMAPMA) microgel particles was shown at elevated temperatures exceeding the volume phase transition temperature (VPTT). The subsequent electrostatic interaction of the poly(NIPAM-co-DMAPMA) microgel matrix with tyrosinase was examined at different adsorption regimes. A considerable increase in the amount of the adsorbed enzyme was detected when the microgel film is first brought into a collapsed state but then was allowed to interact with the enzyme at T < VPTT. Spongelike approach to enzyme adsorption was applied for modification of screen-printed graphite electrodes by poly(NIPAM-co-DMAPMA)/tyrosinase films and the resultant biosensors for phenol were tested amperometrically. By temperature-induced stimulating both (i) poly(NIPAM-co-DMAPMA) microgel adsorption at T > VPTT and (ii) following spongelike tyrosinase loading at T < VPTT, we can achieve more than 3.5-fold increase in biosensor sensitivity for phenol assay. Thus, a very simple, novel, and fast strategy for physical entrapment of biomolecules by the polymeric matrix was proposed and tested. Being based on this unique stimuli-sensitive behavior of the microgel, this stimulated spongelike adsorption provides polymer films comprising concentrated biomaterial.
- Добавил в систему: Сиголаева Лариса Викторовна