HPLC-UV Method for Determing Flavonoids in Hawthorn Flowers and Leaves

Sagaradze, V.A.; Babaeva, E.Y.; Kalenikova, E.I.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 30 августа 2017 г.

Авторы: Sagaradze V.A., Babaeva E.Yu, Kalenikova E.I.
Журнал: Pharmaceutical Chemistry Journal
Том: 51
Номер: 4
Год издания: 2017
Издательство: Kluwer Academic/Plenum Publishers
Местоположение издательства: United States
Первая страница: 277
Последняя страница: 280
DOI: 10.1007/s11094-017-1597-0
Аннотация: An HPLC technique with UV spectrophotometric detection was developed for qualitative and quantitative determination of vitexin, rutin, hyperoside, and quercetin in Crataegus sanguinea hawthorn herbs (flowers and leaves). The separation used a Luna C18(2) column (4.6 × 150 mm, 5 μm) and a stepwise gradient. Calibration curves for the four flavonoids exhibited good linearity (r² > 0.995) in the studied ranges. The analytical recoveries over one day/week were 95.9-104.3%/95.6 – 102.9% for vitexin; 94.0 – 102.2%/93.5 – 101.5% for rutin; 98.0 – 102.2%/97.5 – 102.9% for hyperoside; and 96.0 – 109.8%/97.2 – 111.0% for quercetin. The intra- and interday coefficients of variation (RSD, %) were <3%. The relative error (ε, %) of a single determination at confidence level 95% was <3% for rutin, hyperoside, and vitexin and <4% for quercetin.
Добавил в систему: Каленикова Елена Игоревна

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HPLC-UV Method for Determing Flavonoids in Hawthorn Flowers and Leavesстатья

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