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Current Knowledge of Base Editing and Prime Editingстатья
- Авторы: Averina O.A., Kuznetsova S.A., Permyakov O.A., Sergiev P.V.
- Журнал: Molecular Biology
- Том: 58
- Номер: 4
- Год издания: 2024
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 571
- Последняя страница: 587
- DOI: 10.1134/s0026893324700195
- Аннотация: Modern genetic engineering technologies, such as base editing and prime editing (PE), haveproven to provide the efficient and reliable genome editing tools that obviate the need for donor templates anddouble-strand breaks (DSBs) introduced in DNA. Relatively new, they quickly gained recognition for theiraccuracy, simplicity, and multiplexing capabilities. The review summarizes the new literature on the technol-ogies and considers their architecture, methods to create editors, specificity, efficiency, and versatility.Advantages and disadvantages of the editors are discussed along with their prospective use in basic andapplied research. The review may be useful for planning genome editing studies and analyzing their results tosolve various problems of fundamental biology, biotechnology, medicine, and agriculture.Modern genetic engineering technologies, such as base editing and prime editing (PE), haveproven to provide the efficient and reliable genome editing tools that obviate the need for donor templates anddouble-strand breaks (DSBs) introduced in DNA. Relatively new, they quickly gained recognition for theiraccuracy, simplicity, and multiplexing capabilities. The review summarizes the new literature on the technol-ogies and considers their architecture, methods to create editors, specificity, efficiency, and versatility.Advantages and disadvantages of the editors are discussed along with their prospective use in basic andapplied research. The review may be useful for planning genome editing studies and analyzing their results tosolve various problems of fundamental biology, biotechnology, medicine, and agriculture.
- Добавил в систему: Антохина Оксана Геннадьевна