Аннотация:Zwitterionic stationary phases have unique selectivity due to the presence of both positively and negatively charged groups in the structure of the functional layer. These resins are applied for determining different classes of polar substances in hydrophilic interaction liquid chromatography (HILIC). Most HILIC stationary phases are still based on silica having limited chemical stability. The attachment of zwitterions to the surface of the polymer substrate, such as poly(styrene-divinylbenzene) would expand the utilizing area of these sorbents, including suppressed ion chromatography (IC). Moreover, aromatic substrate and hydrophobic parts of the functional layer would ensure the retention of non-polar compounds in reversed-phase liquid chromatography (RP-HPLC). In this work, novel mixed-mode stationary phases based on modified polymer substrates with attached zwitterionic polyelectrolytes formed by the reaction of 1,4-butanediol diglycidyl ether (1,4-BDDGE) and iminodiacetic acid were obtained. Additional positively charged polyelectrolytes formed by the reaction of 1,4-BDDGE and dimethylamine were grafted for obtaining resins suitable for IC. The application possibilities of the synthesized stationary phases were demonstrated in three modes of liquid chromatography for separating compounds of different charge and polarity. It was demonstrated that grafting positively charged chains leads to an increase in the hydrophilicity of stationary phase in HILIC mode allowing the determination of water-soluble vitamins, amino acids, sugars, nucleobases and nucleosides. The separation of fat-soluble vitamins, as well as alkyl-, chloro- and nitro-phenols using zwitterionic resins in RP-HPLC mode was shown, while in IC mode polyelectrolyte-grafted phases were applied for simultaneous separation of inorganic anions and phosphonic acids.
