Prolidase is a critical enzyme for complete gliadin digestion in Tenebrio molitor larvae

Tereshchenkova, V.F.; Goptar, I.A.; Zhuzhikov, D.P.; Belozersky, M.A.; Dunaevsky, Y.E.; Oppert, B.; Filippova, I.Y.; Elpidina, E.N.

Авторы: Tereshchenkova V.F., Goptar I.A., Zhuzhikov D.P., Belozersky M.A., Dunaevsky Y.E., Oppert B., Filippova I.Yu, Elpidina E.N.
Журнал: Archives of Insect Biochemistry and Physiology
Том: 95
Номер: 4
Год издания: 2017
Издательство: John Wiley & Sons Inc.
Местоположение издательства: United States
Первая страница: e21395
DOI: 10.1002/arch.21395
Аннотация: Prolidase is a proline specific metallopeptidase that cleaves imidodipeptides with C-terminal Pro residue. Prolidase was purified and characterized from the Tenebrio molitor larval midgut. The enzyme was localized in the soluble fraction of posterior midgut tissues, corresponding to a predicted cytoplasmic localization of prolidase according to the structure of the mRNA transcript. Expression of genes encoding prolidase and the major digestive PSP – dipeptidyl peptidase 4, were similar. The pH-optimum of T. molitor prolidase was 7.5, and the enzyme was inhibited by Z-Pro, indicating that it belongs to type I prolidases. In mammals, prolidase is particularly important in the catabolism of a proline-rich protein – collagen. We propose that T. molitor larval prolidase is a critical enzyme for the final stages of digestion of dietary proline-rich gliadins, providing hydrolysis of imidodipeptides in the cytoplasm of midgut epithelial cells. We propose that the products of hydrolysis are absorbed from the luminal contents by peptide transporters, which we have annotated in the T. molitor larval gut transcriptome. The origin of prolidase substrates in the insect midgut is discussed in the context of overall success of grain feeding insects.
Добавил в систему: Элпидина Елена Николаевна

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ЦЭМИ РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ЦЭМИ РАН

Prolidase is a critical enzyme for complete gliadin digestion in Tenebrio molitor larvaeстатья

Прикрепленные файлы