|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Electrical pulse stimulation decreases electrochemical Na+ and K+ gradients in C2C12 myotubesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 16 ноября 2017 г.
- Авторы: Danilov Kirill, Sidorenko Svetlana, Milovanova Kseniya, Klimanova Elizaveta, Kapilevich Leonid V., Orlov Sergei N.
- Журнал: Biochemical and Biophysical Research Communications
- Том: 493
- Номер: 2
- Год издания: 2017
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 875
- Последняя страница: 878
- DOI: 10.1016/j.bbrc.2017.09.133
- Аннотация: Abstract Electrical pulse stimulation (EPS)-treated cultured myotubes are widely employed as an in vitro model of muscle contraction. Here we examined time-dependent EPS action and dose-dependent ouabain action on [Na+]i and [K+]i in C2C12 myotubes. After 2 h of EPS (40 V, 1 Hz, 10 ms) [Na+]i increased by ∼150% whereas [K+]i declined by ∼20%. 3 μM ouabain had a negligible impact on [Na+]i and [K+]i in control cells but increased the [Na+]i/[K+]i ratio in EPS-treated myotubes by 85%. Thus, our results show for the first time that EPS results in dissipation of Na+ and K+ gradients in cultured myotubes and suggest that the augmented production of endogenous cardiotonic steroids may contribute to elevation of the [Na+]i/[K+]i ratio in exercising muscle.
- Добавил в систему: Климанова Елизавета Андреевна