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Peculiarities of luminescent response of bacillus subtilis recombinant strain bearing cloned vibro harveyi lux ab genes to the action of thermostable blood serum compoundsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 сентября 2017 г.
- Авторы: Karimov I.F., Deryabin D.G., Manukhov I.V., Kotova V.Y., Omel'chenko D.O.
- Журнал: Applied Biochemistry and Microbiology
- Том: 46
- Номер: 9
- Год издания: 2010
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 845
- Последняя страница: 848
- DOI: 10.1134/S000368381009005X
- Аннотация: A strain of Bacillus subtilis previously used as the test-organism in bacteriological and nephelometry methods for detection of thrombocytes cation protein (TCP) has been transformed by a plasmid pLFlux containing cloned luxAB genes of a sea luminescent bacterium Vibro harveyi. The designed luminescent biosensor B. subtilis of The All-Russia Collection of Industrial Microorganisms (Moscow) B-10191 demonstrated specific response to the thermostable fraction of guinea pig blood serum. Sensitivity to chromatographically purified TCP as part of the system has been analyzed and the correlation between luminescence inhibition and direct bacterial effect on the target cells has been demonstrated. The obtained results are considered as the first stage of the design of the bioluminescent technology for TCP detection in biological liquids with complicate composition of the components.
- Добавил в систему: Омельченко Денис Олегович