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Changes in spectral properties and composition of lipofuscin fluorophores from human retinal pigment epithelium with age and pathologyстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 марта 2017 г.
- Авторы: Feldman Tatiana B., Yakovleva Marina A., Arbukhanova Patimat M., Borzenok Sergey A., Kononikhin Alexey S., Popov Igor A., Nikolaev Evgeny N., Ostrovsky Mikhail A.
- Журнал: Analytical and Bioanalytical Chemistry
- Том: 407
- Номер: 4
- Год издания: 2015
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 1075
- Последняя страница: 1088
- DOI: 10.1007/s00216-014-8353-z
- Аннотация: Fundus autofluorescence mostly originates from bisretinoid fluorophores in lipofuscin granules, which accumulate in retinal-pigment-epithelium cells with age. The dynamics of accumulation, photo-oxidation, and photodegradation of bisretinoids during aging or in the presence of pathology have been insufficiently investigated. Changes in spectral properties and composition of human lipofuscin-granule fluorophores with age and pathology have now been investigated by a high-performance liquid chromatography method using spectrophotometric and fluorescent detectors connected in series. It was found that: (i) N-retinylidene-N-retinylethanolamine (A2E) fluorescence intensity is not predominant in the chloroform extract of human-cadaver-eye retinal pigment epithelium studied; bisretinoid photo-oxidation and photodegradation products have much higher fluorescent properties; (ii) the relative emission maximum in the fluorescence spectrum of suspended retinal-pigment-epithelium cells obtained from an individual human-cadaver eye without pathology is irrespective of donor age and falls within the range 575 ± 15 nm; in two cadaver eyes with signs of age-related macular degeneration, emission maxima were shifted by 23–36 nm towards the shortwave region; and (iii) the ratio of bisretinoid photo-oxidation and photodegradation products to unoxidized bisretinoids in the chloroform extract of cadaver-eye retinal pigment epithelium increases with donor age, from 0.69 ± 0.03 to 1.32 ± 0.04. The differences in fluorescence properties between chloroform extracts obtained from cadaver eyes with and without signs of age-related macular degeneration could be used to increase the potential of fundus autofluorescence imaging as a noninvasive diagnostic method.
- Добавил в систему: Фельдман Татьяна Борисовна