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Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridizationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 16 декабря 2014 г.
- Авторы: Rebrikov D.V., Britanova O.V., Gurskaya N.G., Lukyanov K.A., Tarabykin V.S., Lukyanov S.A.
- Журнал: Nucleic Acids Research
- Том: 28
- Номер: 20
- Год издания: 2000
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: E90
- Аннотация: Suppression subtractive hybridization (SSH) is one of the most powerful and popular methods for isolating differentially expressed transcripts. However, SSH-generated libraries typically contain some background clones representing non-differentially expressed transcripts. To overcome this problem we developed a simple procedure that substantially decreases the number of background clones. This method is based on the following difference between target and background cDNAs: each kind of background molecule has only one orientation with respect to the two different flanking adapter sequences used in SSH, while truly differentially expressed target cDNA fragments are represented by both sequence orientations. The described method selects the molecules that arose due to hybridization of such mirror-orientated molecules. The efficiency of this method was demonstrated in both model and real experimental subtractions.
- Добавил в систему: Ребриков Денис Владимирович